[leish-l] Fwd: Articles found by RefScout 13/07/05 - 28/2005
jeffreyj at usp.br
jeffreyj at usp.br
Thu Jul 21 09:36:03 BRT 2005
Date: Wed, 13 Jul 2005 03:16:59
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This is RefScout-Newsletter 28/2005.
REQUEST: [ leishmaniasis ]
(16 articles match this request)
PMID: 15979656
TITLE: Time-specific ecological niche modeling predicts spatial dynamics of
vector insects and human dengue cases.
AUTHORS: A Townsend Peterson, Carmen MartÃnez-Campos, Yoshinori Nakazawa,
Enrique MartÃnez-Meyer
AFFILIATION: Natural History Museum and Biodiversity Research Center, University
of Kansas, Lawrence, Kansas 66045, USA.
REFERENCE: Trans R Soc Trop Med Hyg 2005 Sep 99(9):647-55
Numerous human diseases-malaria, dengue, yellow fever and leishmaniasis
, to name a few-are transmitted by insect vectors with brief life cycles
and biting activity that varies in both space and time. Although the
general geographic distributions of these epidemiologically important
species are known, the spatiotemporal variation in their emergence and
activity remains poorly understood. We used ecological niche modeling
via a genetic algorithm to produce time-specific predictive models of
monthly distributions of Aedes aegypti in Mexico in 1995. Significant
predictions of monthly mosquito activity and distributions indicate that
predicting spatiotemporal dynamics of disease vector species is
feasible; significant coincidence with human cases of dengue indicate
that these dynamics probably translate directly into transmission of
dengue virus to humans. This approach provides new potential for
optimizing use of resources for disease prevention and remediation via
automated forecasting of disease transmission risk.
PMID: 15975718
TITLE: Importance of Lutzomyia longipalpis in the dynamics of transmission of
canine visceral leishmaniasis in the endemic area of Porteirinha Municipality,
Minas Gerais, Brazil.
AUTHORS: João C França-Silva, Ricardo A Barata, Roberto T da Costa, Erika
Michalsky Monteiro, George L L Machado-Coelho, Edvá P Vieira, Aluisio Prata,
Wilson Mayrink, Evaldo Nascimento, Consuelo L Fortes-Dias, Jaime C da Silva,
Edelberto S Dias
AFFILIATION: Departamento de Parasitologia, Universidade Federal de Minas
Gerais, Av. Antônio Carlos 6627, CEP 31270-901, Belo Horizonte, MG, Brazil.
REFERENCE: Vet Parasitol 2005 Aug 131(3-4):213-20
A study of Lutzomyia longipalpis (Lutz and Neiva, 1912) (Diptera:
Psychodidae), the primary vector of American visceral leishmaniasis (AVL
), and the canine form of the disease, was carried out in Porteirinha.
The city is situated in the northern part of the Brazilian State of
Minas Gerais and is an endemic area of AVL. Systematic phlebotomine
captures were performed in seven districts with previously reported
cases of canine visceral leishmaniasis, during 2 years (January 2000-
December 2001). A total of 2328 specimens of L. longipalpis were
captured. The association between the local climate variables and the
population density of L. longipalpis was evaluated and rainfall was
determined to be a major factor, with increased populations during the
rainy season (October-March). At the same time period, blood samples
from every dog domiciled in the same seven districts, in total 14,077
animals, were analyzed for infection by viscerotropic Leishmania using
indirect immunofluorescence assay (IFA). Accumulated incidence rates of
canine VL per district varied from 3.40 to 14.34 for the 2-year period.
A positive correlation between the population density of L. longipalpis
and the canine cases of visceral leishmaniasis in Porteirinha was
observed.
PMID: 15967576
TITLE: Canine visceral leishmaniasis in São José de Ribamar, Maranhão State,
Brazil.
AUTHORS: K S Guimarães, Z S Batista, E L Dias, R M S N C Guerra, A D C Costa, A
S Oliveira, K S Calabrese, F O Cardoso, C S F Souza, T Zaverucha do Vale, S C
Gonçalves da Costa, A L Abreu-Silva
AFFILIATION: Curso de Especialização em Ciências Básicas e da Saúde UFMA,
Brazil.
REFERENCE: Vet Parasitol 2005 Aug 131(3-4):305-9
Here, we describe the situation of canine visceral leishmaniasis in two
villages of São José de Ribamar in Maranhão State/Brazil, where human
cases have been registered. Blood samples of 36 household crossbred
dogs from Sergio Tamer village and 43 dogs from Quinta village were
collected and the serum used for serological diagnosis. An Indirect
Fluorescent Antibody Test (IFAT) and enzyme-linked immunosorbent assay (
ELISA) were used to detect antibodies against Leishmania. The clinical
examination showed that 25% of the canine population of Quinta presented
a poor body condition and in 39%, ectoparasites (ticks and fleas) were
detected. In both tests, serology revealed that 21% (9 out of 43) of the
dogs presented antibodies against Leishmania (55% were asymptomatic and
45% were symptomatic). In the Vila Sérgio Tamer, 25% (9 out of 36) of
the dogs were seropositive for Leishmania (66.67% were asymptomatic and
33.33% were symptomatic), 33% presented poor body condition, and 22%
have ectoparasites. The clinical signs more frequent were skin lesions.
The statistical analysis showed that there was no statistical difference
(p>0.05) between the seropositivity of the dogs from the two villages.
The same was observed when the clinical signs were compared (p>0.05).
Both villages have favorable conditions to maintain the cycle of
leishmaniasis.
PMID: 16000412
TITLE: Evaluation of PCR for diagnosis of Indian kala-azar and assessment of
cure.
AUTHORS: R Maurya, R K Singh, B Kumar, P Salotra, M Rai, S Sundar
AFFILIATION: Infectious Diseases Research Laboratory, Department of Medicine,
Institute of Medical Sciences, Banaras Hindu University, 6 SK Gupta, Nagar,
Lanka, Varanasi 221 005, India. shyam_vns at sify.com.
REFERENCE: J Clin Microbiol 2005 Jul 43(7):3038-41
This study was done to evaluate PCR with Ld1 primers for the diagnosis
of Indian visceral leishmaniasis (VL) and to assess its role in
prediction of the disease outcome. The PCR assay was performed with DNA
isolated from the peripheral blood of parasitologically confirmed cases
of VL before the initiation of treatment, just after the end of
treatment, and at 3 and 6 months of follow-up. The pretreatment PCR
result was positive for 100 of 101 patients (sensitivity, 99%;
confidence interval [CI], 94 to 100%). None of the 150 negative controls
tested were PCR positive (specificity, 100%; CI, 96.8 to 100%). Of 60
patients who were treated at our center, 51 (85%; CI, 73 to 93%) became
negative immediately after treatment and continued to be negative at 3
and 6 months of follow-up. At the 3-month follow-up, two of the
remaining nine patients were PCR positive, making 58 (96.7%; CI, 87 to
100%) patients PCR negative. At the 6-month follow-up, all patients
became PCR negative. One patient who was PCR negative immediately after
the end of treatment relapsed 11 months later. This limited prospective
study with VL patients suggests that the PCR assay is a highly sensitive
and specific (99% and 100%, respectively) tool for the diagnosis of VL
. In the majority of patients, it can identify a successful disease
outcome; however, its translation into the field setting remains a major
challenge.
PMID: 16002718
TITLE: 18{beta}-Glycyrrhetinic Acid Triggers Curative Th1 Response and Nitric
Oxide Up-Regulation in Experimental Visceral Leishmaniasis Associated with the
Activation of NF-{kappa}B.
AUTHORS: Anindita Ukil, Aruna Biswas, Tapasi Das, Pijush K Das
AFFILIATION: Molecular Cell Biology Laboratory, Indian Institute of Chemical
Biology, Calcutta, India.
REFERENCE: J Immunol 2005 Jul 175(2):1161-9
The efficacy of 18beta-glycyrrhetinic acid (GRA), a pentacyclic
triterpene belonging to the beta-amyrin series of plant origin, was
evaluated in experimental visceral leishmaniasis. GRA is reported to
have antitumor and immunoregulatory activities, which may be
attributable in part to the induction of NO. Indeed, an 11-fold increase
in NO production was observed with 20 muM GRA in mouse peritoneal
macrophages infected with Leishmania donovani promastigotes. In addition
to having appreciable inhibitory effects on amastigote multiplication
within macrophages (IC(50), 4.6 mug/ml), complete elimination of liver
and spleen parasite burden was achieved by GRA at a dose of 50 mg/kg/day
, given three times, 5 days apart, in a 45-day mouse model of visceral
leishmaniasis. GRA treatment resulted in reduced levels of IL-10 and IL-
4, but increased levels of IL-12, IFN-gamma, TNF-alpha, and inducible NO
synthase, reflecting a switch of CD4(+) differentiation from Th2 to Th1
. This treatment is likely to activate immunity, thereby imparting
resistance to reinfection. GRA induced NF-kappaB migration into the
nucleus of parasite-infected cells and caused a diminishing presence of
IkappaB in the cytoplasm. The lower level of cytoplasmic IkappaBalpha in
GRA-treated cells resulted from increased phosphorylation of
IkappaBalpha and higher activity of IkappaB kinase (IKK). Additional
experiments demonstrated that GRA does not directly affect IKK activity
. These results suggest that GRA exerts its effects at some level
upstream of IKK in the signaling pathway and induces the production of
proinflammatory mediators through a mechanism that, at least in part,
involves induction of NF-kappaB activation.
PMID: 16007269
TITLE: What about Th1/Th2 in cutaneous leishmaniasis vaccine discovery?
AUTHORS: A Campos-Neto
AFFILIATION: The Forsyth Institute, Boston, MA, USA.
REFERENCE: Braz J Med Biol Res 2005 Jul 38(7):979-84
The T helper cell type 1 (Th1) response is essential to resist
leishmaniasis, whereas the Th2 response favors the disease. However,
many leishmanial antigens, which stimulate a Th1 immune response during
the disease or even after the disease is cured, have been shown to have
no protective action. Paradoxically, antigens associated with an early
Th2 response have been found to be highly protective if the Th1 response
to them is generated before infection. Therefore, finding disease-
associated Th2 antigens and inducing a Th1 immune response to them using
defined vaccination protocols is an interesting unorthodox alternative
approach to the discovery of a leishmania vaccine.
PMID: 15980336
TITLE: Alteration of fatty acid and sterol metabolism in miltefosine-resistant
Leishmania donovani promastigotes and consequences for drug-membrane
interactions.
AUTHORS: M Rakotomanga, M Saint-Pierre-Chazalet, P M Loiseau
AFFILIATION: Chimiothérapie Antiparasitaire, UMR 8076 CNRS, Faculté de
Pharmacie, Université Paris XI, F-92290, ChÄtenay-Malabry, France.
REFERENCE: Antimicrob Agents Chemother 2005 Jul 49(7):2677-86
Miltefosine (hexadecylphosphocholine [HePC]) is the first orally active
drug approved for the treatment of visceral leishmaniasis. In order to
investigate the biochemical modifications occurring in HePC-resistant (
HePC-R) Leishmania donovani promastigotes, taking into account the lipid
nature of HePC, we investigated their fatty acid and sterol metabolisms
. We found that the content of unsaturated phospholipid alkyl chains was
lower in HePC-R parasite plasma membranes than in those of the wild
type, suggesting a lower fluidity of HePC-R parasite membranes. We also
demonstrated that HePC insertion within an external monolayer was more
difficult when the proportion of unsaturated phospholipids decreased,
rendering the HePC interaction with the external monolayer of HePC-R
parasites more difficult. Furthermore, HePC-R parasite membranes
displayed a higher content of short alkyl chain fatty acids, suggesting
a partial inactivation of the fatty acid elongation enzyme system in
HePC-R parasites. Sterol biosynthesis was found to be modified in HePC-R
parasites, since the 24-alkylated sterol content was halved in HePC-R
parasites; however, this modification was not related to HePC
sensitivity. In conclusion, HePC resistance affects three lipid
biochemical pathways: fatty acid elongation, the desaturase system
responsible for fatty acid alkyl chain unsaturation, and the C-24-
alkylation of sterols.
PMID: 15995268
TITLE: Challenges in the management of visceral leishmaniasis.
AUTHORS: Shyam Sundar, Ashok Kumar
AFFILIATION: Professor, Department of Medicine, Institute of Medical Sciences,
Banaras Hindu University, Varanasi 221 005, (UP), India.
drshyamsundar at hotmail.com.
REFERENCE: Indian Pediatr 2005 Jun 42(6):523-6
PMID: 15928908
TITLE: Leishmaniasis as an opportunistic infection in HIV-infected patients:
determinants of relapse and mortality in a collaborative study of 228 episodes
in a Mediterreanean region.
AUTHORS: F Pasquau, J Ena, R Sanchez, J M Cuadrado, C Amador, J Flores, C
Benito, C Redondo, J Lacruz, V Abril, J Onofre,
AFFILIATION: Department of Internal Medicine-HIV Unit, Marina Baixa Hospital,
Partida de Galandú 5, 03570, Villajoyosa, Alicante, Spain,
pasquau_fra at gva.es.
REFERENCE: Eur J Clin Microbiol Infect Dis 2005 Jun 24(6):411-8
The clinical presentation of visceral leishmaniasis shares similarities
with other geographically specific infectious diseases associated with
AIDS in terms of relapsing course and atypical presentation. However,
visceral leishmaniasis has not, until now, been included in the AIDS
case definition. The aim of this study was to describe the clinical
features and determinants for relapse and case-fatality of visceral
leishmaniasis in HIV-infected patients from a Spanish Mediterranean area
. A chart review was conducted in 16 hospitals in the autonomous
communities of Valencia and Murcia (Spain). From 1988 to 2001, a total
of 228 episodes of visceral leishmaniasis were diagnosed in 155 HIV-
infected patients by the detection of amastigotes in bone marrow
aspirates or in other tissue samples. Most patients had advanced HIV
disease, with a median CD4+ lymphocyte cell count of 55 cells x 10(9) l
, and 56% of them had a previous AIDS-indicator disease. The median
duration of follow-up was 8.4 months. HIV-infected patients with
visceral leishmaniasis presented with fever (76%), hepatomegaly (77%),
splenomegaly (78%), and varying degrees of cytopenias. Leishmania was
detected in atypical sites in 22 (14%) patients. A total of 37 (24%)
patients had a relapse of visceral leishmaniasis. Female gender was a
risk factor for relapse, whereas administration of secondary prophylaxis
for visceral leishmaniasis and a completed therapy for visceral
leishmaniasis were protective factors against relapse. A total of 86 (54
%) patients died. Independent determinants for survival were CD4+
lymphocyte cell count, completed therapy for leishmania, and secondary
prophylaxis for visceral leishmaniasis. The findings show that, in HIV-
infected patients, visceral leishmaniasis occurs in late stages of HIV
disease and often has a relapsing course. Secondary prophylaxis reduces
the risk of relapse. Visceral leishmaniasis in the HIV-infected
population should be included in the CDC clinical category C for the
definition of AIDS in the same way that other geographically specific
opportunistic infections are included.
PMID: 15879084
TITLE: Cutting edge: the SLAM family receptor Ly108 controls T cell and
neutrophil functions.
AUTHORS: Duncan Howie, F Stephen Laroux, Massimo Morra, Abhay R Satoskar, Lucia
E Rosas, William A Faubion, Aimee Julien, Svend Rietdijk, Anthony J Coyle,
Christopher Fraser, Cox Terhorst
AFFILIATION: Division of Immunology, Beth Israel Deaconess Medical Center,
Harvard Medical School, Boston, MA 02215, USA. duncan.howie at path.ox.ac.uk
REFERENCE: J Immunol 2005 May 174(10):5931-5
Ly108, a glycoprotein of the signaling lymphocytic activation molecule
family of cell surface receptors expressed by T, B, NK, and APCs has
been shown to have a role in NK cell cytotoxicity and T cell cytokine
responses. In this study, we describe that CD4(+) T cells from mice with
a targeted disruption of exons 2 and 3 of Ly108 (Ly108(DeltaE2+3))
produce significantly less IL-4 than wild-type CD4(+) cells, as judged
by in vitro assays and by in vivo responses to cutaneous infection with
Leishmania mexicana. Surprisingly, neutrophil functions are controlled
by Ly108. Ly108(DeltaE2+3) mice are highly susceptible to infection with
Salmonella typhimurium, bactericidal activity of Ly108(DeltaE2+3)
neutrophils is defective, and their production of IL-6, IL-12, and TNF-
alpha is increased. The aberrant bactericidal activity by Ly108(DeltaE2+
3) neutrophils is a consequence of severely reduced production of
reactive oxygen species following phagocytosis of bacteria. Thus, Ly108
serves as a regulator of both innate and adaptive immune responses.
PMID: 15879134
TITLE: Control of infection with Leishmania major in susceptible BALB/c mice
lacking the common gamma-chain for FcR is associated with reduced production of
IL-10 and TGF-beta by parasitized cells.
AUTHORS: Udaikumar M Padigel, Jay P Farrell
AFFILIATION: Department of Pathobiology, School of Veterinary Medicine,
University of Pennsylvania, Philadelphia, PA 19104, USA.
REFERENCE: J Immunol 2005 May 174(10):6340-5
Previous studies have shown that the in vitro ligation of FcgammaRs with
IgG-opsonized Leishmania amastigotes promotes IL-10 production by
macrophages. In addition, infection of either BALB/c mice lacking the
common gamma-chain of Fc receptors (FcgammaR(-/-)) or mice genetically
altered to lack circulating Ab (J(H)D) with Leishmania pifanoi results
in reduced and delayed lesion development and a deficit in the
recruitment of inflammatory cells into infected lesions. We show in this
study that FcgammaR(-/-) mice can control infection with Leishmania
major and totally resolve cutaneous lesions. The ability to eventually
control infection is not associated with a reduction in lesion
inflammation or a reduction in the ability of Leishmania to parasitize
cells through week 6 of infection. The immune response in healing
FcgammaR(-/-) mice is associated with a reduction in numbers of cells
producing Th2-type cytokines, including IL-4 and IL-10, but not an
increase in numbers of IFN-gamma-producing cells characteristic of a
dominant Th1-type response. Instead, we observe a reduction in levels of
IL-10 and TGF-beta within infected lesions, including reduced levels of
these cytokines within parasitized macrophages. Together, these results
suggest that uptake of opsonized parasites via FcgammaRs may be a
strong in vivo stimulus for the production of anti-inflammatory
cytokines that play a role in susceptibility to infection.
PMID: 15860278
TITLE: Stage-specific antileishmanial activity of an inhibitor of SIR2 histone
deacetylase.
AUTHORS: Baptiste Vergnes, Laurent Vanhille, Ali Ouaissi, Denis Sereno
AFFILIATION: IRD UR008 Pathogénie des Trypanosomatidés, Institut de Recherche
pour le Développement, Centre IRD de Montpellier, 911 Av. Agropolis, BP 64501,
34394 Montpellier Cedex 5, France.
REFERENCE: Acta Trop 2005 May 94(2):107-15
Silent information regulator 2 (SIR2) proteins are NAD-dependant
deacetylases found in organisms ranging from bacteria to human. In
eukaryotes, these proteins are involved in many biological processes
including transcriptional repression, metabolism, ageing, or apoptosis.
Here, we have shown that Sirtinol, a commercially available inhibitor of
SIR2 deacetylases, significantly inhibits the in vitro proliferation of
Leishmania infantum axenic amastigotes in a dose-dependent manner. This
activity is stage specific since sirtinol did not affect the in vitro
growth of parasite promastigotes. Growth arrest in amastigotes is
associated with genomic DNA fragmentation, a process reminiscent of
apoptosis. Interestingly parasites carrying extra copies of the LmSIR2
gene were less susceptible to the sirtinol mediated cell death.
Altogether, these results constitute novel evidences that Leishmania
SIR2 proteins play a role in the control of the parasite apoptotic
phenomenon.
PMID: 15986599
TITLE: Influence of phospholipid composition on the adjuvanticity and protective
efficacy of liposome-encapsulated Leishmania donovani antigens.
AUTHORS: Tuhina Mazumdar, K Anam, N Ali
AFFILIATION: Infectious Diseases Group, Indian Institute of Chemical Biology, 4,
Raja S. C. Mullick Road, Kolkata 700032, India.
REFERENCE: J Parasitol 2005 Apr 91(2):269-74
In this study, we evaluate the effect of phospholipid on the
adjuvanicity and protective efficacy of liposome vaccine carriers
against visceral leishmaniasis (VL) in a hamster model. Liposomes
prepared with distearyol derivative of L-alpha-phosphatidyl choline (
DSPC) having liquid crystalline transition temperature (Tc) 54 C were as
efficient as dipalmitoyl (DPPC) (Tc 41 C) and dimyristoyl (DMPC) (Tc 23
C) derivatives in their ability to entrap Leishmania donovani membrane
antigens (LAg) and to potentiate strong antigen-specific antibody
responses. However, whereas LAg in DPPC and DMPC liposomes stimulated
inconsistent delayed type hypersensitivity (DTH) responses, strong DTH
was observed with LAg in DSPC liposomes. The heightened adjuvant
activity of DSPC liposomes corresponded with 95% protection, with almost
no protectivity with LAg in DPPC and DMPC liposomes, 4 mo after
challenge with L. donovani. These data demonstrate the superiority of
DSPC liposomes for formulation of L. donovani vaccine. In addition, they
demonstrate a correlation of humoral and cell-mediated immunity with
protection against VL in hamsters.
PMID: 15972797
TITLE: Thermolytic CpG-containing DNA oligonucleotides as potential
immunotherapeutic prodrugs.
AUTHORS: Andrzej Grajkowski, Joao Pedras-Vasconcelos, Vivian Wang, Cristina
AusÃn, Sonja Hess, Daniela Verthelyi, Serge L Beaucage
AFFILIATION: Laboratory of Chemistry, Center for Drug Evaluation and Research,
Food and Drug Administration 8800 Rockville Pike, Bethesda, MD 20892, USA.
REFERENCE: Nucleic Acids Res 2005 33(11):3550-60
A CpG-containing DNA oligonucleotide functionalized with the 2-(N-formyl
-N-methyl)aminoethyl thiophosphate protecting group (CpG ODN fma1555)
was prepared from phosphoramidites 1a-d using solid-phase techniques.
The oligonucleotide behaved as a prodrug by virtue of its conversion to
the well-studied immunomodulatory CpG ODN 1555 through thermolytic
cleavage of the 2-(N-formyl-N-methyl)aminoethyl thiophosphate protecting
group. Such a conversion occurred at 37 degrees C with a half-time of
73 h. The immunostimulatory properties of CpG ODN fma1555 were evaluated
in two in vivo assays, one of which consisted of mice challenged in the
ear with live Leishmania major metacyclic promastigotes. Local
intradermal administration of CpG ODN fma1555 was as effective as that
of CpG ODN 1555 in reducing the size of Leishmania lesions over time. In
a different infectious model, CpG ODN 1555 prevented the death of
Tacaribe-infected mice (43% survival) when administered between day 0
and 3 post infection. Administration of CpG ODN fma1555 three days
before infection resulted in improved immunoprotection (60-70% survival
). Moreover, co-administration of CpG ODN fma1555 and CpG ODN 1555 in
this model increased the window for therapeutic treatment against
Tacaribe virus infection, and thus supports the use of thermolytic
oligonucleotides as prodrugs in the effective treatment of infectious
diseases.
PMID: 15996442
TITLE: Epidemiology of travel-related hospitalization.
AUTHORS: Shmuel Stienlauf, Gad Segal, Yechezkel Sidi, Eli Schwartz
REFERENCE: J Travel Med 2005 May-Jun 12(3):136-41
Background:: Limited information exists on causes of hospitalization in
patients returning from the tropics, and most is focused on febrile
diseases. We evaluated all causes of post-travel hospitalization in a
tertiary care hospital in Israel. Methods:: Demographics, diagnoses, and
destinations of patients admitted between January 1999 and December
2003 with a history of recent travel were recorded. Demographics and
destination of healthy travelers presenting to our pretravel clinic at
the same period were recorded. Results:: Of 211 patients admitted, 71%
were males, 8% were immigrants/foreign workers, and febrile diseases
accounted for 77% of admissions. The most common diagnoses were malaria
in 54 (26%), unidentified febrile disease in 34 (16%), and dengue fever
in 27 (13%). New World cutaneous leishmaniasis was the most common cause
of admission among nonfebrile patients (18 [9%]). Diarrheal diseases
accounted for only 11% of admissions. Regarding destination, 101 (48%)
patients had been to Asia, 71 (34%) to Africa, and 43 (20%) to the
Americas. Of our healthy traveler population, 59% traveled to Asia, 20%
to Africa, and 20% to the Americas. Travel to Africa carried the highest
risk of being hospitalized (OR 1.85, 95% CI 1.16-2.97; p = .01). Most (
59%) patients returning from Africa had malaria. The principal health
problem originating in Asia was dengue fever (27%), and from Latin
America, cutaneous leishmaniasis (48%). Males comprised 71% of the
patients, and 59% of the healthy traveler population (p < .0001).
Males were more likely to acquire malaria (OR 2.15, 95% CI 1.13-4.09; p
= .02) and leishmaniasis (OR 3.41, 95% CI 0.97-11.89; p = .05).
Conclusions:: Febrile diseases were the most common cause for
hospitalization, with malaria, unidentified febrile diseases, and dengue
fever being the most common. Diseases were destination related; travel
to Africa was associated with a higher rate of hospitalization. Malaria
and cutaneous leishmaniasis had a substantially male predominance,
probably due to risk-taking behavior.
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The following references are revised files and are brought to you in accordance
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PMID: 13528097
TITLE: [Study of 19 biopsies of cutaneous leishmaniasis in Mexico.]
AUTHORS: F MARROQUIN, F BIAGI
REFERENCE: Rev Latinoam Anat Patol 1957 Jul-Dec 1(2):145-50
REQUEST: [ leishmania ]
(12 articles match this request. 7 articles matching other requests removed)
PMID: 15964042
TITLE: Modulation of Leishmania donovani infection and cell viability by
testosterone in bone marrow-derived macrophages: Signaling via surface binding
sites.
AUTHORS: Limin Liu, W Peter M Benten, Lianyun Wang, Xiangfang Hao, Qiaoli Li,
Huaping Zhang, Dongxing Guo, Yajing Wang, Frank Wunderlich, Zhongdong Qiao
AFFILIATION: Medical School, Shanghai Jiaotong University, Huashanlu 1954,
200030 Shanghai, PR China.
REFERENCE: Steroids 2005 Aug 70(9):604-14
Androgens can increase susceptibility toward numerous parasitic
infections as well as modulate apoptosis of immune cells. According to
the current view, androgens mediate immune cell activities not only
through classical intracellular androgen receptors (AR), but also
through membrane receptors on the cell surface. Here, using murine bone
marrow-derived macrophages (BMMs), we examined the influence of
testosterone on Leishmania donovani infection and cell viability in
vitro as well as the possible mechanisms. Our data demonstrated that
testosterone directly increased intramacrophage infection by L. donovani
. In addition, testosterone decreased cell viability by way of apoptosis
, accompanied by increased Fas, FasL, and Caspase-8 expression. However
, these effects of testosterone could not be associated with the
classical AR in BMMs since AR was not detectable using different
experimental techniques. Instead, it was found that testosterone could
bind to the surface of BMMs by the use of an impermeable testosterone-
BSA-FITC in confocal laser scanning microscopy and flow cytometry.
Collectively, our data indicated that the influence of testosterone on L
. donovani infection and viability of BMMs was mediated through the
binding sites of testosterone on cell surfaces, which provided a novel
mode of direct action of testosterone on AR-free BMMs.
PMID: 15998317
TITLE: Unconventional secretory routes: direct protein export across the plasma
membrane of Mammalian cells.
AUTHORS: Walter Nickel
AFFILIATION: Heidelberg University Biochemistry Center (BZH), Im Neuenheimer
Feld 328, 69120 Heidelberg, Germany, walter.nickel at urz.uni-heidelberg.de.
REFERENCE: Traffic 2005 Aug 6(8):607-14
The vast majority of extracellular proteins are exported from mammalian
cells by the endoplasmic reticulum/Golgi-dependent secretory pathway.
For poorly understood reasons, however, a heterogenous group of
extracellular proteins has been discovered that does not make use of
signal peptide-dependent secretory transport. Both the release
mechanisms and the molecular identity of the secretory machines involved
have remained elusive. Recent studies now have established a subgroup
of unconventional secretory proteins capable of translocating from the
cytoplasm directly across the plasma membrane to get access to the
exterior of eukaryotic cells. This review aims to focus on a detailed
comparison of the subcellular site of membrane translocation of various
unconventional secretory proteins such as the proangiogenic molecule
fibroblast growth factor-2 (FGF-2) and Leishmania hydrophilic acylated
surface protein B (HASP B). A potential link between membrane
translocation and quality control as an integral part of unconventional
secretory processes is discussed.
PMID: 15936761
TITLE: Characterisation of a Leishmania mexicana knockout lacking guanosine
diphosphate-mannose pyrophosphorylase.
AUTHORS: James Stewart, Joan Curtis, Timothy P Spurck, Thomas Ilg, Attila
Garami, Tracey Baldwin, Nathalie Courret, Geoffrey I McFadden, Antony Davis,
Emanuela Handman
AFFILIATION: The University of Melbourne, Parkville, Vic. 3052, Australia.
REFERENCE: Int J Parasitol 2005 Jul 35(8):861-73
In eukaryotes, the enzyme GDP-mannose pyrophosphorylase (GDP-MP) is
essential for the formation of GDP-mannose, the donor of activated
mannose for all glycosylation reactions. Unlike other eukaryotes, where
deletion of GDP-mannose pyrophosphorylase is lethal, deletion of this
gene in Leishmania mexicana has no effect on viability, but leads to the
generation of avirulent parasites. In this study, we show that the null
mutants have a perturbed morphology and cytokinesis, retarded growth
and increased adherence to the substratum where they form large colonies
. The null mutants attach avidly to mouse macrophages, but unlike the
wild type organisms, they do not bind to the complement receptor 3 and
are slow to induce phagocytosis. Once internalised, they localise to the
phagolysosome, but in contrast to wild type organisms which transform
into the intracellular amastigote and establish in the macrophage, they
are cleared by 24h in culture and by 5h in vivo. The null mutants are
hypersensitive to human but not mouse complement and to temperature and
acidic pH. Surprisingly, in view of the lack of several known host-
protective antigens, injection of the mutant parasites into BALB/c mice
confers significant and long lasting protection against infection,
suggesting that these temperature sensitive mutants are an attractive
candidate for a live attenuated vaccine.
PMID: 16002669
TITLE: Phenotype and homing of CD4 tumor-specific T cells is modulated by tumor
bulk.
AUTHORS: Fabio Benigni, Valérie S Zimmermann, Stephanie Hugues, Stefano
Caserta, Veronica Basso, Laura Rivino, Elizabeth Ingulli, Laurent Malherbe,
Nicolas Glaichenhaus, Anna Mondino
AFFILIATION: Cancer Immunotherapy and Gene Therapy Program, S. Raffaele
Scientific Institute, Milan, Italy.
REFERENCE: J Immunol 2005 Jul 175(2):739-48
Technical difficulties in tracking endogenous CD4 T lymphocytes have
limited the characterization of tumor-specific CD4 T cell responses.
Using fluorescent MHC class II/peptide multimers, we defined the fate of
endogenous Leishmania receptor for activated C kinase (LACK)-specific
CD4 T cells in mice bearing LACK-expressing TS/A tumors. LACK-specific
CD44(high)CD62L(low) CD4 T cells accumulated in the draining lymph nodes
and had characteristics of effector cells, secreting IL-2 and IFN-gamma
upon Ag restimulation. Increased frequencies of CD44(high)CD62L(low)
LACK-experienced cells were also detected in the spleen, lung, liver,
and tumor itself, but not in nondraining lymph nodes, where the cells
maintained a naive phenotype. The absence of systemic redistribution of
LACK-specific memory T cells correlated with the presence of tumor.
Indeed, LACK-specific CD4 T cells with central memory features (IL-2(+)
IFN-gamma(-)CD44(high)CD62L(high) cells) accumulated in all peripheral
lymph nodes of mice immunized with LACK-pulsed dendritic cells and after
tumor resection. Together, our data demonstrate that although tumor-
specific CD4 effector T cells producing IFN-gamma are continuously
generated in the presence of tumor, central memory CD4 T cells
accumulate only after tumor resection. Thus, the continuous stimulation
of tumor-specific CD4 T cells in tumor-bearing mice appears to hinder
the systemic accumulation of central memory CD4 T lymphocytes.
PMID: 15922377
TITLE: Leishmanicidal cycloartane-type triterpene glycosides from Astragalus
oleifolius.
AUTHORS: Meltem Ozipek, Ali A Dönmez, Ihsan CaliÅ, Reto Brun, Peter Rüedi,
Deniz Tasdemir
AFFILIATION: Department of Pharmacognosy, Faculty of Pharmacy, Hacettepe
University, Ankara, Turkey. meltemozipek at yahoo.com
REFERENCE: Phytochemistry 2005 May 66(10):1168-73
Two new cycloartane-type glycosides oleifoliosides A (1) and B (2) were
isolated from the lower stem parts of Astragalus oleifolius. Their
structures were identified as 3-O-[beta-xylopyranosyl-(1 --> 2)-alpha-
arabinopyranosyl]-6-O-beta-xylopyranosyl-3beta,6alpha,16beta,24(S),25-
pentahydroxycycloartane and 3-O-[beta-xylopyranosyl-(1 --> 2)-alpha-
arabinopyranosyl]-6-O-beta-glucopyranosyl-3beta,6alpha,16beta,24(S),25-
pentahydroxycycloartane, respectively, by means of spectroscopic methods
(IR, 1D and 2D NMR, ESI-MS). Three known cycloartane glycosides
cyclocanthoside E (3), astragaloside II (4) and astragaloside IV (5)
were also isolated and characterized. All five compounds were evaluated
for in vitro trypanocidal, leishmanicidal and antiplasmodial activities
as well as their cytotoxic potential on primary mammalian (L6) cells.
Except for the compound 5, all compounds showed notable growth
inhibitory activity against Leishmania donovani with IC50 values ranging
from 13.2 to 21.3 microg/ml. Only weak activity against Trypanosoma
brucei rhodesiense was observed with the known compounds astragaloside
II (4, IC50 66.6 microg/ml) and cyclocanthoside E (3, IC50 85.2 microg/
ml), while all compounds were inactive against Trypanosoma cruzi and
Plasmodium falciparum. None of the compounds were toxic to mammalian
cells (IC50's > 90 microg/ml). This is the first report of
leishmanicidal and trypanocidal activity of cycloartane-type triterpene
glycosides.
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